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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
10/07/2012 |
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Data da última atualização: |
03/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
YOSHIARA, L. Y.; MADEIRA, T. B.; RIBEIRO, M. L. L.; MANDARINO, J. M. G.; CARRÃO-PANIZZI, M. C.; IDA, E. I. |
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Afiliação: |
L.Y. YOSHIARA, UEL; T.B. MADEIRA, UEL; M.L.L. RIBEIRO, UEL; JOSE MARCOS GONTIJO MANDARINO, CNPSO; MERCEDES CONCORDIA CARRAO PANIZZI, CNPT; ELZA IOUKO IDA, UEL. |
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Título: |
B-glucosidase activity of soybean (Glycine max) embryonic axis germinated in the presence or absence of light. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Food Biochemistry, v. 36, n. 6, p. 699-705, Dec. 2012. |
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DOI: |
doi:10.1111/j.1745-4514.2011.00585.x |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to investigate the b-glucosidase activity of radicles, hypocotyls, cotyledons and epicotyls of soybeans (Glycine max) grown in the presence and absence of light and to obtain an alternative source of b-glucosidase. Seeds were germinated in the presence and absence of light at various time points, and the radicles, epicotyls, hypocotyls and cotyledons were separated. The enzymatic activity in the epicotyls presented the highest activity at 144 h in the presence of light. Specifically, the enzymatic activity of the epicotyls was sixfold higher than that of radicles and 12-fold higher than that of cotyledons. Interestingly, the specific activity of hypocotyls and cotyledons was 4.02-fold and 5.17-fold lower than that of epicotyls after 144 h of germination under light. The high specific activity of lightgerminated epicotyls suggested that the purity of b-glucosidase from epicotyls is greater than b-glucosidase obtained fromother parts of the plant. PRACTICAL APPLICATIONS: Isoflavones are beneficial to human health. Specifically, isoflavones and estrogen have similar positive effects on the symptoms of menopause and osteoporosis. Moreover, isoflavones possess antioxidant and antitumoral properties against certain cancers. In nature, the most common form of the isoflavone is the glycosylated form. Studies on the bioavailability of isoflavones indicate that these compounds behave similarly to estrogen in most biological systems and the aglycone forms aremore bioavailable than their glycoside forms. b-glucosidase is produced from the metabolism of fungi, bacteria or soybeans, and is the only enzyme capable of hydrolyzing isoflavone glycosides to aglycones. Therefore, safe and effective methods for the synthesis of b-glucosidase must be developed to produce soy-based foods with high concentrations of aglycone isoflavones. Thus, the objective of the present study is to investigate the best conditions under which the greatest amount of b-glucosidase could be isolated from soybean. MenosThe aim of this study was to investigate the b-glucosidase activity of radicles, hypocotyls, cotyledons and epicotyls of soybeans (Glycine max) grown in the presence and absence of light and to obtain an alternative source of b-glucosidase. Seeds were germinated in the presence and absence of light at various time points, and the radicles, epicotyls, hypocotyls and cotyledons were separated. The enzymatic activity in the epicotyls presented the highest activity at 144 h in the presence of light. Specifically, the enzymatic activity of the epicotyls was sixfold higher than that of radicles and 12-fold higher than that of cotyledons. Interestingly, the specific activity of hypocotyls and cotyledons was 4.02-fold and 5.17-fold lower than that of epicotyls after 144 h of germination under light. The high specific activity of lightgerminated epicotyls suggested that the purity of b-glucosidase from epicotyls is greater than b-glucosidase obtained fromother parts of the plant. PRACTICAL APPLICATIONS: Isoflavones are beneficial to human health. Specifically, isoflavones and estrogen have similar positive effects on the symptoms of menopause and osteoporosis. Moreover, isoflavones possess antioxidant and antitumoral properties against certain cancers. In nature, the most common form of the isoflavone is the glycosylated form. Studies on the bioavailability of isoflavones indicate that these compounds behave similarly to estrogen in most biological systems and the aglycone forms arem... Mostrar Tudo |
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Palavras-Chave: |
Isoflavona. |
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Categoria do assunto: |
-- |
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Marc: |
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024 7 $adoi:10.1111/j.1745-4514.2011.00585.x$2DOI
100 1 $aYOSHIARA, L. Y.
245 $aB-glucosidase activity of soybean (Glycine max) embryonic axis germinated in the presence or absence of light.$h[electronic resource]
260 $c2012
520 $aThe aim of this study was to investigate the b-glucosidase activity of radicles, hypocotyls, cotyledons and epicotyls of soybeans (Glycine max) grown in the presence and absence of light and to obtain an alternative source of b-glucosidase. Seeds were germinated in the presence and absence of light at various time points, and the radicles, epicotyls, hypocotyls and cotyledons were separated. The enzymatic activity in the epicotyls presented the highest activity at 144 h in the presence of light. Specifically, the enzymatic activity of the epicotyls was sixfold higher than that of radicles and 12-fold higher than that of cotyledons. Interestingly, the specific activity of hypocotyls and cotyledons was 4.02-fold and 5.17-fold lower than that of epicotyls after 144 h of germination under light. The high specific activity of lightgerminated epicotyls suggested that the purity of b-glucosidase from epicotyls is greater than b-glucosidase obtained fromother parts of the plant. PRACTICAL APPLICATIONS: Isoflavones are beneficial to human health. Specifically, isoflavones and estrogen have similar positive effects on the symptoms of menopause and osteoporosis. Moreover, isoflavones possess antioxidant and antitumoral properties against certain cancers. In nature, the most common form of the isoflavone is the glycosylated form. Studies on the bioavailability of isoflavones indicate that these compounds behave similarly to estrogen in most biological systems and the aglycone forms aremore bioavailable than their glycoside forms. b-glucosidase is produced from the metabolism of fungi, bacteria or soybeans, and is the only enzyme capable of hydrolyzing isoflavone glycosides to aglycones. Therefore, safe and effective methods for the synthesis of b-glucosidase must be developed to produce soy-based foods with high concentrations of aglycone isoflavones. Thus, the objective of the present study is to investigate the best conditions under which the greatest amount of b-glucosidase could be isolated from soybean.
653 $aIsoflavona
700 1 $aMADEIRA, T. B.
700 1 $aRIBEIRO, M. L. L.
700 1 $aMANDARINO, J. M. G.
700 1 $aCARRÃO-PANIZZI, M. C.
700 1 $aIDA, E. I.
773 $tJournal of Food Biochemistry$gv. 36, n. 6, p. 699-705, Dec. 2012.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 15 | |
| 2. |  | MOREIRA, A. A.; SANTOS, R. F.; MANDARINO, J. M. G.; VARÉA, G. S.; IDA, E. I.; RIBEIRO, M. L. L. Conversion of isoflavone glucosides to aglycones in whole soybean flour thermally treated and with endogenous B-glucosidase of soybean. In: WORLD CONGRESS OF FOOD SCIENCE AND TECHNOLOGY, 16.; LATIN AMERICAN SEMINAR OF FOOD SCIENCE AND TECHNOLOGY, 17., 2012, Foz do Iguaçu. Addressing global food security and wellness through food science and technology: [proceedings]. Foz do Iguaçu: IUFoST, 2012. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 4. | | YOSHIARA, L. Y.; IDA, E. I.; RIBEIRO, M. L. L.; MANDARINO, J. M. G.; CARRÃO-PANIZZI, M. C. Effect of germination on Beta-glucosidase activity of soybeans hypocotyls and cotyledons. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 22., 2010, Salvador. Ciência e tecnologia de alimentos: potencialidades, desafios e inovações. [S.l.]: SBCTA, 2010. p. 240. CD-DROM. CBCTA.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 5. | | YOSHIARA, L. Y.; MADEIRA, T. B.; RIBEIRO, M. L. L.; MANDARINO, J. M. G.; PANIZZI, M. C. C.; IDA, E. I. Beta-glucosidase activity of soybean epicotyls in germination. In: CONGRESSO INTERNACIONAL DE BIOPROCESSOS NA INDÚSTRIA DE ALIMENTOS, 4.; ENCONTRO REGIONAL SUL DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 10., 2010, Curitiba. [Resumo...]. Curitiba: CIETEP, 2010. Doc. 22. 1 CD-ROM. ICBF 2010/XERSCTA.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 6. | | YOSHIARA, L. Y.; MADEIRA, T. B.; MIRANDA, A. L.; RIBEIRO, M. L. L.; MANDARINO, J. M. G.; CARRÃO-PANIZZI, M. C.; IDA, E. I. Beta-glucosidase activity of soybean radicles in germination. In: WORLD CONGRESS OF FOOD SCIENCE AND TECHNOLOGY, 15., 2010, Cape Town. Food science solutions in an evolving world: abstracts. South Africa: SAAFoST, 2010. Poster 697. CD-ROM. IUFoST 2010.| Biblioteca(s): Embrapa Soja. |
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| 7. | | YOSHIARA, L. Y.; MADEIRA, T. B.; RIBEIRO, M. L. L.; MANDARINO, J. M. G.; CARRÃO-PANIZZI, M. C.; IDA, E. I. B-glucosidase activity of soybean (Glycine max) embryonic axis germinated in the presence or absence of light. Journal of Food Biochemistry, v. 36, n. 6, p. 699-705, Dec. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Soja. |
| |
| 8. | | RIBEIRO, M. L. L.; MANDARINO, J. M. G.; CARRÃO-PANIZZI, M. C.; OLIVEIRA, M. C. N. de; CAMPO, C. B. H.; NEPOMUCENO, A. L.; IDA, E. I. Isoflavone content and ß-glucosidase activity in soybean cultivars of different maturity groups. Journal of Food Composition and Analysis, v. 20, n. 1, p.19-24, Feb. 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Soja. |
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| 9. | | GRADE, L. C.; MOREIRA, A. A.; VAREA, G. S.; MANDARINO, J. M. G.; SILVA, J. B.; IDA, E. I.; RIBEIRO, M. L. L. Immobilized soybean B-glucosidase application in commercial soy drink. In: AMERICAS: INTERNATIONAL CONFERENCE ON SOYBEAN UTILIZATION, 2013, Bento Gonçalves. Proceedings... Brasília, DF: Embrapa, 2013. 1 CD-ROM. 5 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 10. | | GRADE, L. C.; MOREIRA, A. A.; VAREA, G. da S.; MANDARINO, J. M. G.; SILVA, J. B. da; IDA, E. I.; RIBEIRO, M. L. L. Soybean B-Glucosidase immobilisated on chitosan beads and its applicaation in soy drink increase the Aglycones. Brazilian Archives of Biology and Technology, Curitiba, v. 57, n. 5, p. 766-773, Sept./Oct. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 11. | | RIBEIRO, M. L. L.; MANDARINO, J. M. G.; CARRÃO-PANIZZI, M. C.; OLIVEIRA, M. C. N.; CAMPO, C. B. H.; NEPOMUCENO, A. L.; IDA, E. I. ß-glucosidase activity and isoflavone content in germinated soybean radicles and cotyledons. Journal of Food Biochemistry, Westport, v. 30, n. 4, p. 453-465, Aug. 2006. Nome correto do quinto autor HOFFMANN-CAMPO, C.B.| Biblioteca(s): Embrapa Soja. |
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| 12. | | RIBEIRO, M. L. L.; ZIDOI, L.; MANDARINO, J. M. G.; CARRÃO-PANIZZI, M. C.; OLIVEIRA, M. C. N. de; CAMPO, C. B. H.; NEPOMUCENO, A. L.; IDA, E. I. Atividade de b-glicosidases de diferentes cultivares e grupos de maturação de duas safras de soja do Paraná. In: CONGRESSO BRASILEIRO DE SOJA, 4., 2006, Londrina. Resumos... Londrina: Embrapa Soja, 2006. p. 53-54. Organizado por Odilon Ferreira Saraiva, Simone Ery Grosskopf. Nome correto do sexto autor HOFFMANN-CAMPO, C.B.| Biblioteca(s): Embrapa Soja. |
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| 13. | | RIBEIRO, M. L. L.; ZIDOI, L.; MANDARINO, J. M. G.; CARRÃO-PANIZZI, M. C.; OLIVEIRA, M. C. N. de; CAMPO, C. B. H.; NEPOMUCENO, A. L.; IDA, E. I. Isoflavonas da soja de diferentes cultivares e grupos de maturação. In: CONGRESSO BRASILEIRO DE SOJA, 4., 2006, Londrina. Resumos... Londrina: Embrapa Soja, 2006. p. 54. Organizado por Odilon Ferreira Saraiva, Simone Ery Grosskopf. Nome correto do sexto autor HOFFMANN-CAMPO, C.B.| Biblioteca(s): Embrapa Soja. |
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| 14. | | SANTOS, R. F.; OLIVEIRA, C. F.; VARÉA, G. S.; ORRADI DA SILVA, M. L. C.; IDA, E. I.; MANDARINO, J. M. G.; CARRÃO-PANIZZI, M. C.; RIBEIRO, M. L. L. Purification and characterization of soy cotyledon B-glucosidase. Journal of Food Biochemistry, v. 37, n. 3. p. 302-312, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Trigo. |
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| 15. | | SANTOS, R. F.; OLIVEIRA, C. F.; VARÉA, G. S.; SILVA, M. L. C. ORRADI da; IDA, E. I.; MANDARINO, J. M. G.; CARRÃO-PANIZZI, M. C.; RIBEIRO, M. L. L. Purification and characterization of soy cotyledon B-glucosidase. Journal of Food Biochemistry, v. 37, n. 3, p. 302-312, Jun. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 15 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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